Fig. 8. SDS-PAGE of pulldown of WM266-4 cell lysates incubated with biotinylated analogs of 2155-14 pre-complexed with streptavidin beads. (A) WM266-4 cell lysates incubated for 24 h. (B) M14 cell lysates incubated for 24 h. (C) WM266-4 cell lysates incubated for 1 h. Note that 1 h incubation is missing band 1 present in 24 h incubation experiments in either WM266-4 or M14 cells. Note that bands 1-4 appear in both tested melanoma cell lines suggesting a common target for 2155-14. (D) Repeat pulldown experiment with 2529-7 confirms bands 1-4, but not bands 5-6. Bands 1-4 were excised and sent for identification. Pulldown experiment was repeated three times. Protein identification was performed twice on bands from two independent pulldown experiments.